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Chapitre D'ouvrage Année : 2020

Measurements of compensatory endocytosis by antibody internalization and quantification of endocytic vesicle distribution in adrenal chromaffin cells

Mara Ceridono
  • Fonction : Auteur
Sylvette Chasserot-Golaz
  • Fonction : Auteur
Nicolas Vitale
Stéphane Gasman

Résumé

Plasma membrane proteins are amenable to endocytosis assays since they are easily labeled by reagents applied in the extracellular medium. This has been widely exploited to study constitutive endocytosis or ligand-induced receptor endocytosis. Compensatory endocytosis is the mechanism by which components of secretory vesicles are retrieved after vesicle fusion with the plasma membrane in response to cell stimulation and a rise in intracellular calcium. Luminal membrane proteins from secretory vesicles are therefore transiently exposed at the plasma membrane. Here we described an antibody-based method to monitor compensatory endocytosis in chromaffin cells and present an image-based analysis to quantify endocytic vesicles distribution.
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hal-03366447 , version 1 (05-10-2021)

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  • HAL Id : hal-03366447 , version 1

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Mara Ceridono, Sylvette Chasserot-Golaz, Nicolas Vitale, Stéphane Gasman, Stéphane Ory. Measurements of compensatory endocytosis by antibody internalization and quantification of endocytic vesicle distribution in adrenal chromaffin cells. Methods in Molecular Biology: exocytosis and endocytosis, pp.43-51, 2020. ⟨hal-03366447⟩

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