YAP protects the eye from uveitic glaucoma by preserving retinal and anterior chamber homeostases
Juliette Bitard
(1, 2)
,
Elodie-Kim Grellier
(2)
,
Sophie Lourdel
(2)
,
Helena Filipe
(3)
,
Annaïg Hamon
(2, 4)
,
François Fenaille
(5, 6, 7, 8, 9)
,
Florence Castelli
(5, 6, 7, 9, 8, 10, 11, 12, 13)
,
Emeline Chu-Van
(5, 7, 14, 8, 13, 10, 9, 6, 11, 12)
,
Jérôme E. Roger
(2, 1)
,
Morgane Locker
(1)
,
Muriel Perron
(1)
1
NeuroPSI -
Institut des Neurosciences Paris-Saclay
2 CERTO - Centre d'Etudes et de Recherche Thérapeutique en Ophtalmologie
3 ULISBOA - Universidade de Lisboa = University of Lisbon = Université de Lisbonne
4 Irset - Institut de recherche en santé, environnement et travail
5 CEA - CEA- Saclay
6 LI-MS - Laboratoire Innovations en Spectrométrie de Masse pour la santé
7 MTS - Médicaments et Technologies pour la Santé
8 SPI - Service de Pharmacologie et Immunoanalyse
9 MetaboHUB-IDF
10 MetaboHUB
11 DRF (CEA) - Direction de Recherche Fondamentale (CEA)
12 JOLIOT - Institut des Sciences du Vivant Frédéric JOLIOT
13 Université Paris-Saclay
14 SMART-OMICS - Small molecule analysis and omics
2 CERTO - Centre d'Etudes et de Recherche Thérapeutique en Ophtalmologie
3 ULISBOA - Universidade de Lisboa = University of Lisbon = Université de Lisbonne
4 Irset - Institut de recherche en santé, environnement et travail
5 CEA - CEA- Saclay
6 LI-MS - Laboratoire Innovations en Spectrométrie de Masse pour la santé
7 MTS - Médicaments et Technologies pour la Santé
8 SPI - Service de Pharmacologie et Immunoanalyse
9 MetaboHUB-IDF
10 MetaboHUB
11 DRF (CEA) - Direction de Recherche Fondamentale (CEA)
12 JOLIOT - Institut des Sciences du Vivant Frédéric JOLIOT
13 Université Paris-Saclay
14 SMART-OMICS - Small molecule analysis and omics
Juliette Bitard
- Fonction : Auteur
- PersonId : 1347963
- ORCID : 0000-0001-6626-3522
François Fenaille
- Fonction : Auteur
- PersonId : 1136732
- IdHAL : francois-fenaille
- ORCID : 0000-0001-6787-4149
- IdRef : 089320964
Florence Castelli
- Fonction : Auteur
- PersonId : 1326713
- IdHAL : florence-castelli
- ORCID : 0000-0002-6886-8549
Emeline Chu-Van
- Fonction : Auteur
- PersonId : 1341876
- IdHAL : emeline-chu-van
- ORCID : 0000-0003-1827-2138
Jérôme E. Roger
- Fonction : Auteur
- PersonId : 823287
- ORCID : 0000-0002-5061-230X
- IdRef : 112933432
Muriel Perron
- Fonction : Auteur
- PersonId : 741136
- IdHAL : muriel-perron
- ORCID : 0000-0002-1558-8236
- IdRef : 08846847X
Résumé
The role of the transcription coactivator YAP has been studied during eye development but its implication in ocular homeostasis and diseases is much less documented. YAP is expressed by several ocular cell types including the non-pigmented cells of the ciliary body (CB) as well as Müller glial cells (MGs) of the neuroretina. Here, we assessed whether postnatal Yap deletion in these two cell types could affect eye homeostasis during aging.
Methods : Specific Yap deletion in CB and MGs was induced in a conditional mouse model (Yap cKO) at P10. The phenotype was investigated by western-blot, immunohistochemistry, and RNA-sequencing (RNA-seq). Blood defects were identified by fluorescein angiography. Optic nerve degeneration was assessed by optical coherence tomography. Intraocular pressure (IOP) was recorded by a rebound tonometer. Imaging of the CB in healthy and uveitic human patients was performed by ultrasound biomicroscopy.