Revealing the elasticity of an individual aortic fiber during ageing at nanoscale by in situ atomic force microscopy †
Alexandre Berquand
(1)
,
Amandine Wahart
(2)
,
Aubéri Henry
(2)
,
Laetitia Gorisse
(2)
,
Pascal Maurice
(2)
,
Sébastien Blaise
(2)
,
Béatrice Romier-Crouzet
(2)
,
Christine Pietrement
(2)
,
Amar Bennasroune
(2)
,
Hervé Sartelet
(2)
,
Stéphane Jaisson
(2)
,
Philippe Gillery
(2)
,
Laurent Martiny
(2)
,
Fatouma Touré
(3)
,
Laurent Duca
(2)
,
Michael Molinari
(4)
Alexandre Berquand
- Fonction : Auteur
- PersonId : 1359094
- ORCID : 0000-0002-3863-8048
- IdRef : 069921245
Béatrice Romier-Crouzet
- Fonction : Auteur
- PersonId : 797985
- ORCID : 0000-0002-2568-5312
Stéphane Jaisson
- Fonction : Auteur
- PersonId : 793760
Philippe Gillery
- Fonction : Auteur
- PersonId : 756480
- ORCID : 0000-0002-1100-8759
Laurent Duca
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- Fonction : Auteur correspondant
- PersonId : 1057572
- ORCID : 0000-0002-6035-4906
- IdRef : 084595701
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Michael Molinari
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- Fonction : Auteur correspondant
- PersonId : 760543
- ORCID : 0000-0001-9906-655X
- IdRef : 070428220
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Résumé
Arterial stiffness is a complex process affecting the aortic tree that significantly contributes to cardiovascular diseases (systolic hypertension, coronary artery disease, heart failure or stroke). This process involves a large extracellular matrix remodeling mainly associated with elastin content decrease and collagen content increase. Additionally, various chemical modifications that accumulate with ageing have been shown to affect long-lived assemblies, such as elastic fibers, that could affect their elasticity. To precisely characterize the fiber changes and the evolution of its elasticity with ageing, high resolution and multimodal techniques are needed for precise insight into the behavior of a single fiber and its surrounding medium. In this study, the latest developments in atomic force microscopy and the related nanomechanical modes are used to investigate the evolution and in a near-physiological environment, the morphology and elasticity of aorta cross sections obtained from mice of different ages with an unprecedented resolution. In correlation with more classical approaches such as pulse wave velocity and fluorescence imaging, we demonstrate that the relative Young's moduli of elastic fibers, as well as those of the surrounding areas, significantly increase with ageing. This nanoscale characterization presents a new view on the stiffness process, showing that, besides the elastin and collagen content changes, elasticity is impaired at the molecular level, allowing a deeper understanding of the ageing process. Such nanomechanical AFM measurements of mouse tissue could easily be applied to studies of diseases in which elastic fibers suffer pathologies such as atherosclerosis and diabetes, where the precise quantification of fiber elasticity could better follow the fiber remodeling and predict plaque rupture. † Electronic supplementary information (ESI) available. See
Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Revealing the elasticity of an individual aortic fiber during ageing at nanoscale by in situ atomic force microscopy †
|
Résumé |
en
Arterial stiffness is a complex process affecting the aortic tree that significantly contributes to cardiovascular diseases (systolic hypertension, coronary artery disease, heart failure or stroke). This process involves a large extracellular matrix remodeling mainly associated with elastin content decrease and collagen content increase. Additionally, various chemical modifications that accumulate with ageing have been shown to affect long-lived assemblies, such as elastic fibers, that could affect their elasticity. To precisely characterize the fiber changes and the evolution of its elasticity with ageing, high resolution and multimodal techniques are needed for precise insight into the behavior of a single fiber and its surrounding medium. In this study, the latest developments in atomic force microscopy and the related nanomechanical modes are used to investigate the evolution and in a near-physiological environment, the morphology and elasticity of aorta cross sections obtained from mice of different ages with an unprecedented resolution. In correlation with more classical approaches such as pulse wave velocity and fluorescence imaging, we demonstrate that the relative Young's moduli of elastic fibers, as well as those of the surrounding areas, significantly increase with ageing. This nanoscale characterization presents a new view on the stiffness process, showing that, besides the elastin and collagen content changes, elasticity is impaired at the molecular level, allowing a deeper understanding of the ageing process. Such nanomechanical AFM measurements of mouse tissue could easily be applied to studies of diseases in which elastic fibers suffer pathologies such as atherosclerosis and diabetes, where the precise quantification of fiber elasticity could better follow the fiber remodeling and predict plaque rupture. † Electronic supplementary information (ESI) available. See
|
Auteur(s) |
Alexandre Berquand
1
, Amandine Wahart
2
, Aubéri Henry
2
, Laetitia Gorisse
2
, Pascal Maurice
2
, Sébastien Blaise
2
, Béatrice Romier-Crouzet
2
, Christine Pietrement
2
, Amar Bennasroune
2
, Hervé Sartelet
2
, Stéphane Jaisson
2
, Philippe Gillery
2
, Laurent Martiny
2
, Fatouma Touré
3
, Laurent Duca
2
, Michael Molinari
4
1
LRN -
Laboratoire de Recherche en Nanosciences - EA 4682
( 426360 )
- U.F.R. Sciences Exactes et Naturelles
Moulin de la Housse
B.P. 1039
51687 Reims Cedex 2
- France
2
MEDyC -
Matrice Extracellulaire et Dynamique Cellulaire - UMR CNRS 7369
( 57707 )
- UFR Sciences Exactes et Naturelles
Campus Moulin de la Housse
51687 REIMS Cedex 2
FRANCE
- France
3
CRIBL -
Contrôle de la Réponse Immune B et des Lymphoproliférations
( 526216 )
- Centre de Biologie et Recherche en Santé (CBRS)
Rue du Pr. Bernard Descottes 87025 LIMOGES Cedex
- France
4
CBMN -
Chimie et Biologie des Membranes et des Nanoobjets
( 24489 )
- IECB 2, rue Robert Escarpit 33607 PESSAC CEDEX
- France
|
Date de publication |
2021
|
Date de publication électronique |
2020-12-08
|
Volume |
13
|
Numéro |
2
|
Page/Identifiant |
1124-1133
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Domaine(s) |
|
DOI | 10.1039/d0nr06753a |
Origine :
Fichiers produits par l'(les) auteur(s)
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