Distinct spermiogenic phenotypes underlie sperm elimination in the Segregation Distorter meiotic drive system - Archive ouverte HAL
Article Dans Une Revue PLoS Genetics Année : 2021

Distinct spermiogenic phenotypes underlie sperm elimination in the Segregation Distorter meiotic drive system

Résumé

Segregation Distorter (SD) is a male meiotic drive system in Drosophila melanogaster. Males heterozygous for a selfish SD chromosome rarely transmit the homologous SD + chromosome. It is well established that distortion results from an interaction between Sd, the primary distorting locus on the SD chromosome and its target, a satellite DNA called Rsp, on the SD + chromosome. However, the molecular and cellular mechanisms leading to postmeiotic SD + sperm elimination remain unclear. Here we show that SD/SD + males of different genotypes but with similarly strong degrees of distortion have distinct spermiogenic phenotypes. In some genotypes, SD + spermatids fail to fully incorporate protamines after the removal of histones, and degenerate during the individualization stage of spermiogenesis. In contrast, in other SD/SD + genotypes, protamine incorporation appears less disturbed, yet spermatid nuclei are abnormally compacted, and mature sperm nuclei are eventually released in the seminal vesicle. Our analyses of different SD + chromosomes suggest that the severity of the spermiogenic defects associates with the copy number of the Rsp satellite. We propose that when Rsp copy number is very high (> 2000), spermatid nuclear compaction defects reach a threshold that triggers a checkpoint controlling sperm chromatin quality to eliminate abnormal spermatids during individualization.
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Dates et versions

hal-03357914 , version 1 (29-09-2021)

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Marion Herbette, Xiaolu Wei, Ching-Ho Chang, Amanda Larracuente, Benjamin Loppin, et al.. Distinct spermiogenic phenotypes underlie sperm elimination in the Segregation Distorter meiotic drive system. PLoS Genetics, 2021, 17 (7), pp.e1009662. ⟨10.1371/journal.pgen.1009662⟩. ⟨hal-03357914⟩
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