Approaches to monitor ATP levels in living cells: where do we stand? - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEBS Journal Année : 2022

Approaches to monitor ATP levels in living cells: where do we stand?

Résumé

ATP is the most universal and essential energy molecule in cells. This is due to its ability to store cellular energy in form of high-energy phosphate bonds, which are extremely stable and readily usable by the cell. This energy is key for a variety of biological functions such as cell growth and division, metabolism, and signaling, and for the turnover of biomolecules. Understanding how ATP is produced and hydrolyzed with a spatiotemporal resolution is necessary to understand its functions both in physiological and in pathological contexts. In this review, first we will describe the organization of the electron transport chain and ATP synthase, the main molecular motor for ATP production in mitochondria. Second, we will review the biochemical assays currently available to estimate ATP quantities in cells, and we will compare their readouts, strengths, and weaknesses. Finally, we will explore the palette of genetically encoded biosensors designed for microscopy-based approaches, and show how their spatiotemporal resolution opened up the possibility to follow ATP levels in living cells.
Fichier principal
Vignette du fichier
Ley-Ngardigal et al_2021_Approaches to monitor ATP levels in living cells_R2_clean_figures.pdf (3.5 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03355070 , version 1 (30-09-2021)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Seyta Ley-Ngardigal, Giulia Bertolin. Approaches to monitor ATP levels in living cells: where do we stand?. FEBS Journal, 2022, 289 (24), pp.7940-7969. ⟨10.1111/febs.16169⟩. ⟨hal-03355070⟩
84 Consultations
114 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More