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Communication Dans Un Congrès Année : 2017

Discovering genes involved in ecological preference in European white oaks using an RNAseq approach

Résumé

The European white oaks are mainly represented by two species: sessile (Quercus petraea Matt. Liebl.) and pedunculate (Quercus robur L.) oaks [1]. The distribution of these two sympatric species, is largely imposed by environmental factors such as light, soil composition and humidity [2]. Sessile oak is generally found on deep, well drained and rather acidic soil, while pedunculate oak favors deep and fertile bottomland with sometime large level of hydromorphia. It is more tolerant to root hypoxia, whereas sessile oak is more drought tolerant [3]. This species complex constitutes therefore a powerful system to determine the role of gene expression in ecological preference. While the role of gene expression in ecological speciation is receiving a growing interest in animals, it still remains in its infancy in forest trees. To fill this gap, we exposed seedlings of these two oak species to a cycle of waterlogging followed by a moderate drought after a recovery period. White roots were harvested on the two species along the whole cycle. An additional sampling of the immersed portion of the stem (i.e containing hypertrophied lenticels) was also performed after the seedlings received the waterlogging treatment. Gene expression for the different time points of the kinetics (5 in total) and tissues was analyzed using an RNAseq approach. Genes showing differential expression between the two species were identified using the Deseq and the EdgeR software. Overall, 851 and 123 genes were found to be over-expressed between pedunculate oak and in sessile oak during waterlogging and drought stress, respectively. Genes regulated by waterlogging and drought stress were validated by qPCR. Our results provide new insights on the role of gene expression in shaping adaptive phenotypes in these two oak species. For instance, we found that the suberin pathway is an important component of waterlogging adaptation in pedunculate oak by favoring O2 diffusion to the root tip.
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Dates et versions

hal-03351960 , version 1 (27-09-2021)

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  • HAL Id : hal-03351960 , version 1

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Grégoire Le Provost, Isabelle Lesur, Céline Lalanne, Karine Labadie, Jean Marc Aury, et al.. Discovering genes involved in ecological preference in European white oaks using an RNAseq approach. Workshop on Fagaceae and Nothofagaceae genetics and genomics, Nov 2017, Shangai, China. ⟨hal-03351960⟩
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