Simultaneous genotyping of snails and infecting trematode parasites using high‐throughput amplicon sequencing - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular Ecology Resources Année : 2022

Simultaneous genotyping of snails and infecting trematode parasites using high‐throughput amplicon sequencing

Résumé

Several methodological issues currently hamper the study of entire trematode communities within populations of their intermediate snail hosts. Here we develop a new workflow using high-throughput amplicon sequencing to simultaneously genotype snail hosts and their infecting trematode parasites. We designed primers to amplify four snail and five trematode markers in a single multiplex PCR. While also applicable to other genera, we focused on medically and economically important snail genera within the superorder Hygrophila and targeted a broad taxonomic range of parasites within the class Trematoda. We tested the workflow using 417 Biomphalaria glabrata specimens experimentally infected with Schistosoma rodhaini, two strains of Schistosoma mansoni and combinations thereof. We evaluated the reliability of infection diagnostics, the robustness of the workflow, its specificity related to host and parasite identification, and the sensitivity to detect co-infections, immature infections and changes of parasite biomass during the infection process. Finally, we investigated its applicability in wild-caught snails of other genera naturally infected with a diverse range of trematodes. After stringent quality control the workflow allows the identification of snails to species level, and of trematodes to taxonomic levels ranging from family to strain. It is sensitive to detect immature infections and changes in parasite biomass described in previous experimental studies. Co-infections were successfully identified, opening the possibility to examine parasite-parasite interactions such as interspecific competition. Together, these results demonstrate that our workflow provides a powerful tool to analyse the processes shaping trematode communities within natural snail populations.
Fichier principal
Vignette du fichier
Hammoud-MolEcolRes-Simultaneous-MANUSCRIT.pdf (1.01 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03347370 , version 1 (17-09-2021)

Identifiants

Citer

Cyril Hammoud, Stephen Mulero, Bert Van Bocxlaer, Jérôme Boissier, Dirk Verschuren, et al.. Simultaneous genotyping of snails and infecting trematode parasites using high‐throughput amplicon sequencing. Molecular Ecology Resources, 2022, 22 (2), pp.567-586. ⟨10.1111/1755-0998.13492⟩. ⟨hal-03347370⟩
58 Consultations
148 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More