In vivo Dominant-Negative Effect of an SCN5A Brugada Syndrome Variant - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Physiology Année : 2021

In vivo Dominant-Negative Effect of an SCN5A Brugada Syndrome Variant

Résumé

Loss-of-function mutations in the cardiac Na + channel α-subunit Na v 1.5, encoded by SCN5A , cause Brugada syndrome (BrS), a hereditary disease characterized by sudden cardiac death due to ventricular fibrillation. We previously evidenced in vitro the dominant-negative effect of the BrS Na v 1.5-R104W variant, inducing retention of wild-type (WT) channels and leading to a drastic reduction of the resulting Na + current ( I Na ). To explore this dominant-negative effect in vivo , we created a murine model using adeno-associated viruses (AAVs). Methods Due to the large size of SCN5A , a dual AAV vector strategy was used combining viral DNA recombination and trans -splicing. Mice were injected with two AAV serotypes capsid 9: one packaging the cardiac specific troponin-T promoter, the 5′ half of hSCN5A cDNA, a splicing donor site and a recombinogenic sequence; and another packaging the complementary recombinogenic sequence, a splicing acceptor site, the 3′ half of hSCN5A cDNA fused to the gfp gene sequence, and the SV40 polyA signal. Eight weeks after AAV systemic injection in wild-type (WT) mice, echocardiography and ECG were recorded and mice were sacrificed. The full-length hSCN5A-gfp expression was assessed by western blot and immunohistochemistry in transduced heart tissues and the Na + current was recorded by the patch-clamp technique in isolated adult GFP-expressing heart cells. Results Almost 75% of the cardiomyocytes were transduced in hearts of mice injected with hNa v 1.5 and ∼30% in hNa v 1.5-R104W overexpressing tissues. In ventricular mice cardiomyocytes expressing R104W mutant channels, the endogenous I Na was significantly decreased. Moreover, overexpression of R104W channels in normal hearts led to a decrease of total Na v 1.5 expression. The R104W mutant also induced a slight dilatation of mice left ventricles and a prolongation of RR interval and P-wave duration in transduced mice. Altogether, our results demonstrated an in vivo dominant-negative effect of defective R104W channels on endogenous ones. Conclusion Using a trans -splicing and viral DNA recombination strategy to overexpress the Na + channel in mouse hearts allowed us to demonstrate in vivo the dominant-negative effect of a BrS variant identified in the N-terminus of Na v 1.5.

Dates et versions

hal-03342597 , version 1 (13-09-2021)

Identifiants

Citer

Nicolas Doisne, Marta Grauso, Nathalie Mougenot, Michel Clergue, Charlotte Souil, et al.. In vivo Dominant-Negative Effect of an SCN5A Brugada Syndrome Variant. Frontiers in Physiology, 2021, 12, ⟨10.3389/fphys.2021.661413⟩. ⟨hal-03342597⟩
20 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More