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Journal Articles ACS Applied Bio Materials Year : 2021

Cryogel-Integrated Biochip for Liver Tissue Engineering


Microfluidic systems and polymer hydrogels have been widely developed for tissue engineering. Yet only a few tools combining both approaches, especially for in vitro liver models, are being explored. In this study, an alginate-based cryogel-integrated biochip was engineered for dynamic hepatoma cell line culture in three dimensions (3D). The alginate cryogel was covalently cross-linked in the biochip at subzero temperatures (T < 0 °C) to create a scaffold with high mechanical stability and an interconnected macroporous network. By varying the alginate concentration and the cross-linker ratio, the Young's modulus of the cryogel can be fine-tuned between 1.5 and 29 kPa, corresponding to the range of stiffness of the different physiological states of the liver. We demonstrated that HepG2/C3A cells can be cultured and maintained viable under dynamic conditions in this device up to 6 days. Albumin synthesis and glucose consumption increased over the cell culture days. Moreover, a 3D cell structure was observed across the entire height of the biochip, which was preserved following alginate lyase treatment to remove the cryogel-based scaffold. In summary, these results represent a proof of concept of an interesting new cell culture technology that should be further investigated to engineer healthy and cirrhotic liver models.
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hal-03322953 , version 1 (20-08-2021)



Lilandra Boulais, Rachid Jellali, Ulysse Pereira, Eric Leclerc, Sidi A Bencherif, et al.. Cryogel-Integrated Biochip for Liver Tissue Engineering. ACS Applied Bio Materials, 2021, 4 (7), pp.5617-5626. ⟨10.1021/acsabm.1c00425⟩. ⟨hal-03322953⟩
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