Article Dans Une Revue Chemical Science Année : 2021

Unprecedented hour-long residence time of a cation in a left-handed G-quadruplex

Résumé

Cations are critical for the folding and assembly of nucleic acids. In G-quadruplex structures, cations can bind between stacked G-tetrads and coordinate with negatively charged guanine carbonyl oxygens. They usually exchange between binding sites and with the bulk in solution with time constants ranging from sub-millisecond to seconds. Here we report the first observation of extremely long-lived K+ and NH4+ ions, with an exchange time constant on the order of an hour, when coordinated at the center of a left-handed G-quadruplex DNA. A single-base mutation, that switched one half of the structure from left- to right-handed conformation resulting in a right–left hybrid G-quadruplex, was shown to remove this long-lived behaviour of the central cation.

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hal-03321839 , version 1 (29-07-2025)

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Fernaldo Richtia Winnerdy, Blaž Bakalar, Poulomi Das, Brahim Heddi, Adrien Marchand, et al.. Unprecedented hour-long residence time of a cation in a left-handed G-quadruplex. Chemical Science, 2021, 12 (20), pp.7151-7157. ⟨10.1039/d1sc00515d⟩. ⟨hal-03321839⟩
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