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Article Dans Une Revue Biochemistry Année : 1998

Dimerization of Native Myosin LC2(RLC)-Free Subfragment 1 from Adult Rabbit Skeletal Muscle

Karima Taouil
  • Fonction : Auteur
Nathalie d'Hahan
  • Fonction : Auteur
Asuncion Aguilar
  • Fonction : Auteur
Zalika Merah
  • Fonction : Auteur
Jean-Pierre Dalbiez
  • Fonction : Auteur
Patrick Bayol
  • Fonction : Auteur
Nathalie Guillo
  • Fonction : Auteur
Louis Patard
  • Fonction : Auteur
Vincent Cabane
  • Fonction : Auteur
Matteo Ferrari
  • Fonction : Auteur
Gonzalo Figuera Picazo
  • Fonction : Auteur
Hanh Dam Hieu
  • Fonction : Auteur
Mathilde Francin

Résumé

We reinvestigated whether the native myosin LC2-free-subfragment 1 (S1) dimer exists by using viscometry, capillary electrophoresis, and laser light scattering. We found that the intrinsic viscosity of the monomer is [eta]m = 6.7 cm3/g and its translation diffusion coefficient is (c = 0) = 4.43 x 10(-)7 cm2/s. For the dimer, [eta]d = 19.8 cm3/g and (c = 0) = 2.54 x 10(-)7 cm2/s. Using the Svedberg equation and introducing the values of the sedimentation coefficients (5.05 S for the monomer and 6.05 S for the dimer), we find the following molecular weights: Mr,m = 108 000 Da and Mr,d = 213 000 Da, which agree well with previous determinations. Capillary electrophoresis successfully separated S1(A1) and S1(A2), in a monomer buffer, and S1(A1) and S1(A2) and a heterodimer S1(A1)-S1(A2), in a dimer buffer. An interesting feature of the monomer-dimer equilibrium is the presence of temperature transitions, whose positions and widths depend upon the buffer conditions. At low temperatures, a pure dimer was observed, whereas at high temperatures only the monomer was present. The dimerization site on both myosin and S1 is extremely labile.

Dates et versions

hal-03299800 , version 1 (26-07-2021)

Identifiants

Citer

Jean-Emile Morel, Karima Taouil, Nathalie d'Hahan, Asuncion Aguilar, Zalika Merah, et al.. Dimerization of Native Myosin LC2(RLC)-Free Subfragment 1 from Adult Rabbit Skeletal Muscle. Biochemistry, 1998, 37 (43), pp.15129 - 15136. ⟨10.1021/bi9804232⟩. ⟨hal-03299800⟩

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