Article Dans Une Revue Journal of Bacteriology Année : 2006

PopF1 and PopF2, Two Proteins Secreted by the Type III Protein Secretion System of Ralstonia solanacearum , Are Translocators Belonging to the HrpF/NopX Family

Damien Meyer
Sébastien Cunnac
Mareva Guéneron
  • Fonction : Auteur
Céline Declercq
  • Fonction : Auteur
Frédérique van Gijsegem
Christian Boucher
  • Fonction : Auteur

Résumé

ABSTRACT Ralstonia solanacearum GMI1000 is a gram-negative plant pathogen which contains an hrp gene cluster which codes for a type III protein secretion system (TTSS). We identified two novel Hrp-secreted proteins, called PopF1 and PopF2, which display similarity to one another and to putative TTSS translocators, HrpF and NopX, from Xanthomonas spp. and rhizobia, respectively. They also show similarities with TTSS translocators of the YopB family from animal-pathogenic bacteria. Both popF1 and popF2 belong to the HrpB regulon and are required for the interaction with plants, but PopF1 seems to play a more important role in virulence and hypersensitive response (HR) elicitation than PopF2 under our experimental conditions. PopF1 and PopF2 are not necessary for the secretion of effector proteins, but they are required for the translocation of AvrA avirulence protein into tobacco cells. We conclude that PopF1 and PopF2 are type III translocators belonging to the HrpF/NopX family. The hrpF gene of Xanthomonas campestris pv. campestris partially restored HR-inducing ability to popF1 popF2 mutants of R. solanacearum , suggesting that translocators of R. solanacearum and Xanthomonas are functionally conserved. Finally, R. solanacearum strain UW551, which does not belong to the same phylotype as GMI1000, also possesses two putative translocator proteins. However, although one of these proteins is clearly related to PopF1 and PopF2, the other seems to be different and related to NopX proteins, thus showing that translocators might be variable in R. solanacearum .

Dates et versions

hal-03295464 , version 1 (22-07-2021)

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Citer

Damien Meyer, Sébastien Cunnac, Mareva Guéneron, Céline Declercq, Frédérique van Gijsegem, et al.. PopF1 and PopF2, Two Proteins Secreted by the Type III Protein Secretion System of Ralstonia solanacearum , Are Translocators Belonging to the HrpF/NopX Family. Journal of Bacteriology, 2006, 188 (13), pp.4903-4917. ⟨10.1128/JB.00180-06⟩. ⟨hal-03295464⟩
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