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Article Dans Une Revue Biomedical Chromatography Année : 2021

Determination of amoxicillin and cotrimoxazole concentrations in sputum of patients with cystic fibrosis

Résumé

In the management of cystic fibrosis, treatments against Staphylococcus aureus and Haemophilus influenzae such as amoxicillin or cotrimoxazole have to be prescribed and the antibiotherapy efficacy may be linked to the concentration that reaches the infected site. As cystic fibrosis patients present disturbed pharmacokinetics parameters, a drug monitoring would be relevant to assess lungs distribution of antibiotics and to optimize dosing regimens. In this context, the aim of the study was to develop and validate HPLC-based methods for the determination of both antibiotics in bronchial sputum from cystic fibrosis patients, in order to assess drugs distribution into lungs. Plasma proteins were precipitated by acetonitrile, amoxicillin concentrations in sputum were determined by HPLC coupled with tandem-mass spectrometry. Following a liquid extraction with ethyl acetate, cotrimoxazole was quantified by HPLC using ultraviolet detection. Both methods were found rapid, specific, accurate and reproducible. The method was applied to patient’s samples. In three treated patients, concentration of amoxicillin in sputum were closed and below the lower limit of quantification (0.1 μg/g) and in six patients, sputum concentrations up to 11.1 μg/g and 6.4 μg/g were measured for sulfamethoxazole and trimethoprim respectively.
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Dates et versions

hal-03282644 , version 1 (08-09-2021)

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Camille Tron, Chantal Belleguic, Caroline Piau, Graziella Brinchault, Eric Deneuville, et al.. Determination of amoxicillin and cotrimoxazole concentrations in sputum of patients with cystic fibrosis. Biomedical Chromatography, 2021, 35 (12), pp.e5208. ⟨10.1002/bmc.5208⟩. ⟨hal-03282644⟩
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