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Article Dans Une Revue Experimental Parasitology Année : 1988

Plasmodium falciparum: Analysis of B epitopes of a polypeptide antigen expressed in Escherichia coli, using monoclonal antibodies

Thierry Blisnick
Fernando Lema
  • Fonction : Auteur
Jean Claude Mazie
  • Fonction : Auteur

Résumé

Monoclonal antibodies were raised against a recombinant molecule corresponding to the polypeptide 72 kDa, previously described as possibly related to protection in Plasmodium falciparum infection. Selection of hybridoma cell lines was done by immunofluorescence to guarantee the reactivity of the monoclonal antibodies both against the recombinant and the native molecule of the parasite. Monoclonal antibodies were characterized by serological and immunochemical techniques. Competitive binding assays between monoclonal antibodies defined four different B epitopes. One epitope is specific for P. falciparum, a second is also present in P. vivax, while the two others seem to be ubiquitous and are also present in the rodent parasite P. chabaudi. The ubiquitous epitope 72.C is apparently the only one recognized by squirrel monkey sera presenting protective antibodies against the asexual blood infection by P. falciparum.

Dates et versions

hal-03246360 , version 1 (02-06-2021)

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Citer

Thierry Blisnick, Fernando Lema, Jean Claude Mazie, Luiz Pereira da Silva. Plasmodium falciparum: Analysis of B epitopes of a polypeptide antigen expressed in Escherichia coli, using monoclonal antibodies. Experimental Parasitology, 1988, 67 (2), pp.247-256. ⟨10.1016/0014-4894(88)90072-0⟩. ⟨hal-03246360⟩

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