Characterisation of PfSec61, a Plasmodium falciparum homologue of a component of the translocation machinery at the endoplasmic reticulum membrane of eukaryotic cells1Note: nucleotide sequence data reported in this paper is available in the Genbank™ database under the accession number AF006204.1 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular and Biochemical Parasitology Année : 1998

Characterisation of PfSec61, a Plasmodium falciparum homologue of a component of the translocation machinery at the endoplasmic reticulum membrane of eukaryotic cells1Note: nucleotide sequence data reported in this paper is available in the Genbank™ database under the accession number AF006204.1

Résumé

Plasmodium falciparum secretes several proteins that cause changes in the erythrocyte membrane enabling it to survive within red blood cells. Little is known of the mechanisms involved in the secretion and targeting of parasite polypeptides to the various cell compartments. The P. falciparum gene homologous to the mammalian Sec61alpha, gene, which encodes a component of the translocation pore in the endoplasmic reticulum of eukaryotic cells, was characterised to investigate the translocation process in the parasite. PfSec61 is present as a unique copy in the parasite genome and was mapped to chromosome 13. It encodes a 40 kDa polypeptide, as shown by immunoblotting and immunoprecipitation of [35S]methionine metabolically-labelled parasite extracts. The deduced amino acid sequence of PfSec61 is 87% similar to the mammalian polypeptide, and the two proteins give similar hydropathy plots. These results strongly suggest that PfSec61 has the same topological orientation and functional role as Sec61alpha. Anti-PfSec61 antibodies were used to investigate the cellular location and kinetics of expression of the polypeptide in the parasite. Immunofluorescence confocal microscopy showed that PfSec61 was located in the parasite cytoplasm, close to the nucleus, in a position consistent with its being in the endoplasmic reticulum.

Dates et versions

hal-03246347 , version 1 (02-06-2021)

Identifiants

Citer

Sandrine Couffin, Rosaura Hernandez-Rivas, Thierry Blisnick, Denise Mattei. Characterisation of PfSec61, a Plasmodium falciparum homologue of a component of the translocation machinery at the endoplasmic reticulum membrane of eukaryotic cells1Note: nucleotide sequence data reported in this paper is available in the Genbank™ database under the accession number AF006204.1. Molecular and Biochemical Parasitology, 1998, 92 (1), pp.89-98. ⟨10.1016/s0166-6851(97)00234-x⟩. ⟨hal-03246347⟩

Collections

PASTEUR CNRS
11 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More