Ionizing-radiation-resistant Kocuria rhizophila PT10 isolated from the Tunisian Sahara xerophyte Panicum turgidum: Polyphasic characterization and proteogenomic arsenal - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Genomics Année : 2021

Ionizing-radiation-resistant Kocuria rhizophila PT10 isolated from the Tunisian Sahara xerophyte Panicum turgidum: Polyphasic characterization and proteogenomic arsenal

Sihem Guesmi
  • Fonction : Auteur
Imen Nouioui
  • Fonction : Auteur
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  • IdRef : 182126196
Afef Najjari
  • Fonction : Auteur
Kais Ghedira
  • Fonction : Auteur
José Igual
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Guylaine Miotello
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Ameur Cherif
Jean Armengaud
Hans-Peter Klenk
  • Fonction : Auteur
Haïtham Sghaier
  • Fonction : Auteur

Résumé

A new strain belonging to the genus Kocuria, designed PT10, was isolated from irradiated roots of the xerophyte Panicum turgidum. Isolate PT10 is a Gram-positive, coccoid, aerobic and ionizing-radiation (IR)-resistant actinobacterium. PT10 has shown an ability to survive under extreme conditions, such as gamma irradiation, desiccation and high concentration of hydrogen peroxide. Phenotypic, chemotaxonomic and comparative genome analyses support the assignment of strain PT10 (LMG 31102 = DSM 108617) as Kocuria rhizophila. The complete genome sequence of PT10 consists of one chromosome (2,656,287 bps), with a 70.7% G + C content and comprises 2481 protein-coding sequences. A total of 1487 proteins were identified by LC-MS/MS profiling. In silico analyses revealed that the proteome of the oxidation-tolerant PT10 possesses several features explaining its IR-resistant phenotype and many adaptive pathways implicated in response to environmental pressures - desiccation, cold, reactive oxygen species and other stressors.

Dates et versions

hal-03237995 , version 1 (26-05-2021)

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Paternité - Pas de modifications

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Sihem Guesmi, Petar Pujic, Imen Nouioui, Audrey Dubost, Afef Najjari, et al.. Ionizing-radiation-resistant Kocuria rhizophila PT10 isolated from the Tunisian Sahara xerophyte Panicum turgidum: Polyphasic characterization and proteogenomic arsenal. Genomics, 2021, 113 (1), pp.317-330. ⟨10.1016/j.ygeno.2020.11.029⟩. ⟨hal-03237995⟩
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