Targeted DNP for biomolecular solid-state NMR
Résumé
High-field dynamic nuclear polarization is revolutionizing the scope of solid-state NMR with new
applications in surface chemistry, materials science and structural biology. In this perspective article, we
focus on a specific DNP approach, called targeted DNP, in which the paramagnets introduced to
polarize are not uniformly distributed in the sample but site-specifically located on the biomolecular
system. After reviewing the various targeting strategies reported to date, including a bio-orthogonal
chemistry-based approach, we discuss the potential of targeted DNP to improve the overall NMR
sensitivity while avoiding the use of glass-forming DNP matrix. This is especially relevant to the study of
diluted biomolecular systems such as, for instance, membrane proteins within their lipidic environment.
We also discuss routes towards extracting structural information from paramagnetic relaxation
enhancement (PRE) induced by targeted DNP at cryogenic temperature, and the possibility to recover
site-specific information in the vicinity of the paramagnetic moieties using high-resolution selective DNP
spectra. Finally, we review the potential of targeted DNP for in-cell NMR studies and how it can be used
to extract a given protein NMR signal from a complex cellular background.
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