The “birth event” is considered as an altered ambiantal situation since it affects woman sensibility and causes a temporary alteration of her birth space perception. The study focuses on a spatial characterization of ambiances and analysis of stress level of woman in labor in the Maternity and Neonatology Center of Tunis-Tunisia, captured through ElectroDermal Activity (EDA). This study highlights correlation between stressful ambiantal situations, architectural aspects of the birth space and altered perception of women in labor. Capturing emotions and analyzing their impact on perceiving spaces is susceptible to renew the way of understanding ambiances.