Article Dans Une Revue FEBS Letters Année : 2003

In vitro DNA-binding properties of VirB, the Shigella flexneri virulence regulatory protein

Christophe Beloin

Résumé

The DNA-binding activity of the Shigella flexneri VirB transcription factor was studied in vitro. The protein was found to bind non-specifically to DNA, but showed preferential binding to VirB-dependent promoter sequences. DNA binding was contingent on the presence of an intact helix-turn-helix motif. While high molecular mass protein-DNA complexes were formed in both specific and non-specific interactions with DNA, mutant derivatives of VirB lacking a leucine zipper domain or a carboxyl-terminal-located oligomerisation domain formed discrete complexes, indicating that an ability to oligomerise on DNA was responsible for the formation of high molecular mass complexes by the wild-type protein.

Fichier principal
Vignette du fichier
McKenna_et_al_01=05=03.pdf (216.76 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Licence

Dates et versions

hal-03217885 , version 1 (10-05-2021)

Licence

Identifiants

Citer

Sorcha Mckenna, Christophe Beloin, Charles J. Dorman. In vitro DNA-binding properties of VirB, the Shigella flexneri virulence regulatory protein. FEBS Letters, 2003, 545 (2-3), pp.183-7. ⟨10.1016/S0014-5793(03)00524-6⟩. ⟨hal-03217885⟩
68 Consultations
121 Téléchargements

Altmetric

Partager

  • More