Instability of the NS1 Glycoprotein from La Reunion 2018 Dengue 2 Virus (Cosmopolitan-1 Genotype) in Huh7 Cells Is Due to Lysine Residues on Positions 272 and 324 - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Molecular Sciences Année : 2021

Instability of the NS1 Glycoprotein from La Reunion 2018 Dengue 2 Virus (Cosmopolitan-1 Genotype) in Huh7 Cells Is Due to Lysine Residues on Positions 272 and 324

Résumé

La Reunion island in the South West Indian Ocean is now endemic for dengue followingthe introduction of dengue virus serotype 2 (DENV-2) cosmopolitan-I genotype in 2017. DENV-2infection causes a wide spectrum of clinical manifestations ranging from flu-like disease to severedengue. The nonstructural glycoprotein 1 (NS1) has been identified as playing a key role in denguedisease severity. The intracellular NS1 exists as a homodimer, whereas a fraction is driven towardsthe plasma membrane or released as a soluble hexameric protein. Here, we characterized the NS1glycoproteins from clinical isolates DES-14 and RUN-18 that were collected during the DENV-2epidemics in Tanzania in 2014 and La Reunion island in 2018, respectively. In relation to hepa-totropism of the DENV, expression of recombinant DES-14 NS1 and RUN-18 NS1 glycoproteins wascompared in human hepatoma Huh7 cells. We observed that RUN-18 NS1 was poorly stable inHuh7 cells compared to DES-14 NS1. The instability of RUN-18 NS1 leading to a low level of NS1secretion mostly relates to lysine residues on positions 272 and 324. Our data raise the issue of theconsequences of a defect in NS1 stability in human hepatocytes in relation to the major role of NS1 inthe pathogenesis of the DENV-2 infection.
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Dates et versions

hal-03215502 , version 1 (03-05-2021)

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Eva Ogire, Olivier Diaz, Pierre-Olivier Vidalain, Vincent Lotteau, Philippe Desprès, et al.. Instability of the NS1 Glycoprotein from La Reunion 2018 Dengue 2 Virus (Cosmopolitan-1 Genotype) in Huh7 Cells Is Due to Lysine Residues on Positions 272 and 324. International Journal of Molecular Sciences, 2021, 22 (4), pp.1951. ⟨10.3390/ijms22041951⟩. ⟨hal-03215502⟩
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