Article Dans Une Revue ACS Applied Materials & Interfaces Année : 2016

pH-Responsive Nanometric Polydiacetylenic Micelles Allow for Efficient Intracellular siRNA Delivery

Résumé

A novel generation of pH-responsive photopolymerized diacetylenic amphiphile (PDA) micelles with a diameter of 10 nm was designed and optimized for the intracellular delivery of siRNAs. Dialysis and photopolymerization of the micelles allowed a strong reduction of the cytotoxicity of the nanovector, while the hydrophilic histidine headgroup permitted enhancing the siRNA delivery potential by improving the endosomal escape via imidazole protonation. These PDA-micellar systems were fully characterized by DLS, TEM, and DOSY-NMR experiments. The resulting bioactive complexes of PDA-micelles with siRNA were shown to have an optimal size below 100 nm.

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hal-03187361 , version 1 (13-05-2024)

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Manon Ripoll, Patrick Neuberg, Antoine Kichler, Nassera Tounsi, Alain Wagner, et al.. pH-Responsive Nanometric Polydiacetylenic Micelles Allow for Efficient Intracellular siRNA Delivery. ACS Applied Materials & Interfaces, 2016, 8 (45), pp.30665-30670. ⟨10.1021/acsami.6b09365⟩. ⟨hal-03187361⟩
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