Metallophyte wastes and polymetallic catalysis: a promising combination in green chemistry. The illustrative synthesis of 59-capped RNA3
Résumé
The compatibility of metallic catalysts derived from plants was studied for the chemical solid-phase synthesis of a high added-value biomolecule: 59-capped RNA (GpppRNA or 7m GpppRNA) involved in many essential biological processes. These molecules are of great interest for biologists for structural and mechanistic studies of their complexes with RNA capping enzymes. Several polymetallic catalysts were prepared from various metallophytes species growing in mine sites in a context of phytoremediation programs. Their catalytic efficacy was evaluated for the coupling reaction of guanosine diphosphate (GDP) with a preactivated 59-monophosphate hexathymidylate (pT 6) or a pRNA to form 59-GpppT 6 or 59-GpppRNA respectively. The direct coupling of 7m GDP with pRNA was also investigated. With Znenriched catalysts obtained from Zn metallophytes, the capping yields were better than those obtained with conventional catalyst ZnCl 2. Interestingly with Ni-enriched plant extracts the yield was higher than with NiCl 2 , a polymetallic catalysis was certainly involved. With this plant-inspired catalysis, metallophytes wastes become efficient and greener polymetallic catalysts. Finally, environmental benefits and effectiveness can be combined.
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