A simple ATAC-seq protocol for population epigenetics [version 1; peer review: 1 approved with reservations] - Archive ouverte HAL Access content directly
Journal Articles Wellcome Open Research Year : 2020

A simple ATAC-seq protocol for population epigenetics [version 1; peer review: 1 approved with reservations]

Abstract

We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size, and it requires little molecular biology handling expertise.
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Dates and versions

hal-03145575 , version 1 (18-02-2021)
hal-03145575 , version 2 (18-02-2021)

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Ronaldo de Carvalho Augusto, Aki Minoda, Christoph Grunau. A simple ATAC-seq protocol for population epigenetics [version 1; peer review: 1 approved with reservations]. Wellcome Open Research, 2020, 5, pp.121. ⟨10.12688/wellcomeopenres.15552.1⟩. ⟨hal-03145575v1⟩
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