A simple ATAC-seq protocol for population epigenetics [version 1; peer review: 1 approved with reservations]
Résumé
We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies. The protocol is a streamlined version of the Assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) that provides a positive display of accessible, presumably euchromatic regions. The protocol is straightforward and can be used with small individuals such as daphnia and schistosome worms, and probably many other biological samples of comparable size, and it requires little molecular biology handling expertise.
Mots clés
epigenetics
epigenomics
ATAC-seq
Daphnia pulex
Schistosoma mansoni Conceptualization
Investigation
Methodology
Writing -Original Draft Preparation
Writing -Review & Editing
Author roles: Augusto RdC : Conceptualization
Conceptualization
Minoda A Grunau C Funding Acquisition
Project Administration
Writing -Review & Editing No competing interests were disclosed
Schistosoma mansoni
Domaines
Evolution [q-bio.PE]| Origine | Fichiers produits par l'(les) auteur(s) |
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