Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms - Archive ouverte HAL Access content directly
Journal Articles Journal of Cell Science Year : 2021

Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms

Francois Iv
Pascale Barbier
Laurie Ramond
Alex Llewellyn
Mayssa Belhabib
Jérôme Wenger
Gijsje Koenderink
Manos Mavrakis

Abstract

Septin GTP-binding proteins contribute essential biological functions that range from the establishment of cell polarity to animal tissue morphogenesis. Human septins in cells form hetero-octameric septin complexes containing the ubiquitously expressed SEPT9. Despite the established role of SEPT9 in mammalian development and human pathophysiology, biochemical and biophysical studies have relied on monomeric SEPT9 thus not recapitulating its native assembly into hetero-octameric complexes. We established a protocol that enabled the first-time isolation of recombinant human septin octamers containing distinct SEPT9 isoforms. A combination of biochemical and biophysical assays confirmed the octameric nature of the isolated complexes in solution. Reconstitution studies showed that octamers with either a long or a short SEPT9 isoform form filament assemblies, and can directly bind and cross-link actin filaments, raising the possibility that septin-decorated actin structures in cells reflect direct actin-septin interactions. Recombinant SEPT9-containing octamers will make it possible to design cell-free assays to dissect the complex interactions of septins with cell membranes and the actin/microtubule cytoskeleton.
Fichier principal
Vignette du fichier
Iv_MainSupp_red.pdf (4.13 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03119193 , version 1 (06-10-2021)

Identifiers

Cite

Francois Iv, Carla Silva Martins, Gerard Castro-Linares, Cyntia Taveneau, Pascale Barbier, et al.. Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms. Journal of Cell Science, 2021, 134 (15), pp.jcs258484. ⟨10.1242/jcs.258484⟩. ⟨hal-03119193⟩
260 View
118 Download

Altmetric

Share

Gmail Facebook X LinkedIn More