Multiphoton imaging of tumor biomarkers in situ using highly oriented conjugates of single-domain antibodies and quantum dots - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2015

Multiphoton imaging of tumor biomarkers in situ using highly oriented conjugates of single-domain antibodies and quantum dots

Résumé

High-quality imaging for immunofluorescence diagnosis in medical practice requires a high sensitivity of labeling and discrimination between the fluorescence of immunostaining probes and tissue autofluorescence. Multiphoton microscopy with excitation in the near-infrared spectral region, far from the region of excitation of tissue autofluorescence, enables deep imaging of biological tissues. CdSe/ZnS quantum dots (QDs) conjugated to single-domain antibodies (sdAbs) are efficient diagnostic nanoprobes with diameters <;12 nm and the two-photon absorption cross section exceeding 49,000 GM. Such nanoprobes ensure clear discrimination of tumor areas from normal tissue with the ratio of sdAb-QD emission to the autofluorescence for two-photon excitation >40 times higher than that for one-photon excitation.
Fichier non déposé

Dates et versions

hal-03112342 , version 1 (16-01-2021)

Identifiants

Citer

Alyona Sukhanova, Hilal Hafian, Michel Pluot, Jacques Cohen, Jean-Marc Millot, et al.. Multiphoton imaging of tumor biomarkers in situ using highly oriented conjugates of single-domain antibodies and quantum dots. 2015 International Conference on Biomedical Engineering and Computational Technologies (SIBIRCON), Oct 2015, Novosibirsk, France. pp.119-121, ⟨10.1109/SIBIRCON.2015.7361864⟩. ⟨hal-03112342⟩
40 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More