The ISO 16300-3 standard considers the required artefacts for interoperability verification and validation throughout the whole cycle of manufacturing application development. In this chapter, an important work of ISO series standards has been elaborated in the context of manufacturing application development. The current work presents the ISO 16300-3 main guidelines devoted to the verification and validation of software unit’s interoperability for the manufacturing software units which can be considered as the most critical part of any automated manufacturing process. The various proposals of ISO 16300-3 are individually detailed to some extent showing its pertinence for a systematic verification and validation of manufacturing software units interoperability.