The phenotype of target pancreatic cancer cells influences cell death by magnetic hyperthermia with nanoparticles carrying gemicitabine and the pseudo-peptide NucAnt - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nanomedicine: Nanotechnology, Biology and Medicine Année : 2019

The phenotype of target pancreatic cancer cells influences cell death by magnetic hyperthermia with nanoparticles carrying gemicitabine and the pseudo-peptide NucAnt

Mourad Sanhaji
  • Fonction : Auteur
Julia Göring
  • Fonction : Auteur
Pierre Couleaud
  • Fonction : Auteur
Antonio Aires
  • Fonction : Auteur
Aitziber Cortajarena
  • Fonction : Auteur
Marcus Stapf
  • Fonction : Auteur
Yuri Volkov
  • Fonction : Auteur
Alfonso Latorre
  • Fonction : Auteur
Álvaro Somoza
  • Fonction : Auteur
Rodolfo Miranda
  • Fonction : Auteur
Ingrid Hilger
  • Fonction : Auteur

Résumé

In this paper we show that conjugation of magnetic nanoparticles (MNPs) with Gemcitabine and/or NucAnt (N6L) fostered their internalization into pancreatic tumor cells and that the coupling procedure did not alter the cytotoxic potential of the drugs. By treating tumor cells (BxPC3 and PANC-1) with the conjugated MNPs and magnetic hyperthermia (43°C, 60 min), cell death was observed. The two pancreatic tumor cell lines showed different reactions against the combined therapy according to their intrinsic sensitivity against Gemcitabine (cell death, ROS production, ability to activate ERK 1/2 and JNK). Finally, tumors (e.g. 3 mL) could be effectively treated by using almost 4.2 × 10 5 times lower Gemcitabine doses compared to conventional therapies. Our data show that this combinatorial therapy might well play an important role in certain cell phenotypes with low readiness of ROS production. This would be of great significance in distinctly optimizing local pancreatic tumor treatments.
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Dates et versions

hal-03095445 , version 1 (04-01-2021)

Identifiants

Citer

Mourad Sanhaji, Julia Göring, Pierre Couleaud, Antonio Aires, Aitziber Cortajarena, et al.. The phenotype of target pancreatic cancer cells influences cell death by magnetic hyperthermia with nanoparticles carrying gemicitabine and the pseudo-peptide NucAnt. Nanomedicine: Nanotechnology, Biology and Medicine, 2019, 20, pp.101983. ⟨10.1016/j.nano.2018.12.019⟩. ⟨hal-03095445⟩

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