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Pré-Publication, Document De Travail Année : 2020

Biomimetic, smart and multivalent ligands for G-quadruplex isolation and bioorthogonal imaging

Thibaut Charbonnier
  • Fonction : Auteur
Pauline Lejault
  • Fonction : Auteur
Joanna Zell
  • Fonction : Auteur
Claire Bernhard
  • Fonction : Auteur
Ibai Valverde
David Monchaud

Résumé

G-quadruplexes (G4s) continue to gather wide attention in the field of chemical biology as their prevalence in the human genome and transcriptome strongly suggests that they may play key regulatory roles in cell biology. G4-specific, cell-permeable small molecules (G4-ligands) innovately permit the interrogation of cellular circuitries in order to assess to what extent G4s influence cell fate and functions. Here, we report on multivalent, biomimetic G4-ligands referred to as TASQs that enable both the isolation and visualization of G4s in human cells. Two biotinylated TASQs, BioTASQ and BioCyTASQ, are indeed efficient molecular tools to fish out G4s of mixtures of nucleic acids through simple affinity capture protocols and to image G4s in cells via a biotin/avidin pretargeted imaging system first applied here to G4s, found to be a reliable alternative to in situ click chemistry.
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Dates et versions

hal-03086822 , version 1 (22-12-2020)

Identifiants

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Francesco Rota Sperti, Thibaut Charbonnier, Pauline Lejault, Joanna Zell, Claire Bernhard, et al.. Biomimetic, smart and multivalent ligands for G-quadruplex isolation and bioorthogonal imaging. 2020. ⟨hal-03086822⟩
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