Modelling of immunosensor response: the evaluation of binding kinetics between an immobilized receptor and structurally-different genetically engineered ligands
Résumé
is about half of that of the mGCSF, which can be explained by the smaller diffusion coefficient of the larger molecule. Moreover, unlike SCF-Lα-GCSF, the (GCSF)2Lα adsorption kinetics cannot be adequately described by the standard Langmuir kinetics model and surface regeneration (induced by 'washing') experiments illustrate that (GCSF)2Lα, unlike the mGCSF and SCF-Lα-GCSF, is irreversibly bound to the surface modified by immobilized GCSF-Receptors. Therefore, to describe binding kinetics in the case of (GCSF)2Lα we have applied advanced kinetic model based on three protein association stages (three-stage kinetics model) in which (GCSF)2Lα forms several different intermediate complexes with GCSF-Receptor. This model precisely describes the time-varying surface concentration of (GCSF)2Lα bound to surface modified by immobilized GCSF-Receptors. In addition to the bioanalytical-aspects possible improvement of GCSF-based drugs is discussed.
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