Transcriptomics at Maize Embryo/Endosperm Interfaces Identifies a Transcriptionally Distinct Endosperm Subdomain Adjacent to the Embryo Scutellum - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue The Plant cell Année : 2020

Transcriptomics at Maize Embryo/Endosperm Interfaces Identifies a Transcriptionally Distinct Endosperm Subdomain Adjacent to the Embryo Scutellum

Nicolas Doll
Jérémy Just
Gwyneth Ingram
Peter Rogowsky

Résumé

A population of endosperm cells adjacent to the embryo scutellum shows transcriptomic enrichment in transport functions and is influenced by embryo development. Seeds are complex biological systems comprising three genetically distinct tissues nested one inside another (embryo, endosperm, and maternal tissues). However, the complexity of the kernel makes it difficult to understand intercompartment interactions without access to spatially accurate information. Here, we took advantage of the large size of the maize (Zea mays) kernel to characterize genome-wide expression profiles of tissues at different embryo/endosperm interfaces. Our analysis identifies specific transcriptomic signatures in two interface tissues compared with whole seed compartments: the scutellar aleurone layer and the newly named endosperm adjacent to scutellum (EAS). The EAS, which appears around 9 d after pollination and persists for around 11 d, is confined to one to three endosperm cell layers adjacent to the embryonic scutellum. Its transcriptome is enriched in genes encoding transporters. The absence of the embryo in an embryo specific mutant can alter the expression pattern of EAS marker genes. The detection of cell death in some EAS cells together with an accumulation of crushed cell walls suggests that the EAS is a dynamic zone from which cell layers in contact with the embryo are regularly eliminated and to which additional endosperm cells are recruited as the embryo grows.
Fichier principal
Vignette du fichier
200201 TPC2019-LSB-00756R1_Merged PDF all files.pdf (1.3 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03065555 , version 1 (21-12-2020)

Licence

Paternité

Identifiants

Citer

Nicolas Doll, Jérémy Just, Véronique Brunaud, José Caïus, Aurélie Grimault, et al.. Transcriptomics at Maize Embryo/Endosperm Interfaces Identifies a Transcriptionally Distinct Endosperm Subdomain Adjacent to the Embryo Scutellum. The Plant cell, 2020, 32 (4), pp.833-852. ⟨10.1105/tpc.19.00756⟩. ⟨hal-03065555⟩
125 Consultations
153 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More