Biological validation of coenzyme Q redox state by HPLC-EC measurement: relationship between coenzyme Q redox state and coenzyme Q content in rat tissues
Résumé
he properties of coenzymes Q (CoQ9 and CoQ10)are closely linked to their redox state (CoQox/total CoQ)·100.In this work, CoQ redox state was biologically validated by highperformance liquid chromatography-electrochemical measure-ment after modulation of mitochondrial electron flow of culturedcells by molecules increasing (rotenone, carbonyl cyanide chlo-rophenylhydrazone) or decreasing (antimycin) CoQ oxidation.The tissue specificity of CoQ redox state and content wereinvestigated in control and hypoxic rats. In control rats, therewas a strong negative linear regression between tissular CoQredox state and CoQ content. Hypoxia increased CoQ9 redoxstate and decreased CoQ9 content in a negative linear relation-ship in the different tissues, except the heart and lung. This resultdemonstrates that, under conditions of mitochondrial impair-ment, CoQ redox control is tissue-specific.