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Article Dans Une Revue FEBS Letters Année : 2004

Biological validation of coenzyme Q redox state by HPLC-EC measurement: relationship between coenzyme Q redox state and coenzyme Q content in rat tissues

A. Galinier
A.M. Bessac
  • Fonction : Auteur
S. Caspar-Bauguil
  • Fonction : Auteur
B. Periquet
  • Fonction : Auteur
M. Comtat
  • Fonction : Auteur
J.P. Thouvenot
  • Fonction : Auteur
L. Casteilla
  • Fonction : Auteur

Résumé

he properties of coenzymes Q (CoQ9 and CoQ10)are closely linked to their redox state (CoQox/total CoQ)·100.In this work, CoQ redox state was biologically validated by highperformance liquid chromatography-electrochemical measure-ment after modulation of mitochondrial electron flow of culturedcells by molecules increasing (rotenone, carbonyl cyanide chlo-rophenylhydrazone) or decreasing (antimycin) CoQ oxidation.The tissue specificity of CoQ redox state and content wereinvestigated in control and hypoxic rats. In control rats, therewas a strong negative linear regression between tissular CoQredox state and CoQ content. Hypoxia increased CoQ9 redoxstate and decreased CoQ9 content in a negative linear relation-ship in the different tissues, except the heart and lung. This resultdemonstrates that, under conditions of mitochondrial impair-ment, CoQ redox control is tissue-specific.

Dates et versions

hal-03059952 , version 1 (13-12-2020)

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Citer

A. Galinier, Audrey Carrière, Y. Fernandez, A.M. Bessac, S. Caspar-Bauguil, et al.. Biological validation of coenzyme Q redox state by HPLC-EC measurement: relationship between coenzyme Q redox state and coenzyme Q content in rat tissues. FEBS Letters, 2004, 578 (1-2), pp.53-57. ⟨10.1016/j.febslet.2004.10.067⟩. ⟨hal-03059952⟩
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