Strategies for bacterial expression of protein–peptide complexes: Application to solubilization of papillomavirus E6 - Archive ouverte HAL Access content directly
Journal Articles Protein Expression and Purification Year : 2011

Strategies for bacterial expression of protein–peptide complexes: Application to solubilization of papillomavirus E6

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Nicole Brimer
  • Function : Author
Yves Nominé
Scott Vande Pol
  • Function : Author
Gilles Travé
  • Function : Author
  • PersonId : 1077169
Katia Zanier
  • Function : Author
  • PersonId : 1077166

Abstract

E6 is a small oncoprotein involved in tumorigenesis induced by papillomaviruses (PVs). E6 often recognizes its cellular targets by binding to short motifs presenting the consensus LXXLL. E6 proteins have long resisted structural analysis. We found that bovine papillomavirus type 1 (BPV1) E6 binds the N-terminal LXXLL motif of the cellular protein paxillin with significantly higher affinity as compared to other E6/peptide interactions. Although recombinant BPV1 E6 was poorly soluble in the free state, provision of the paxillin LXXLL peptide during BPV1 E6 biosynthesis greatly enhanced the protein's solubility. Expression of BPV1 E6/LXXLL peptide complexes was carried out in bacteria in the form of triple fusion constructs comprising, from N-to C-terminus, the soluble carrier protein maltose binding protein (MBP), the LXXLL motif and the E6 protein. A TEV protease cleavage site was placed either between MBP and LXXLL motif or between LXXLL motif and E6. These constructs allowed us to produce highly concentrated samples of BPV1 E6, either covalently fused to the C-terminus of the LXXLL motif (intra-molecular complex) or non-covalently bound to it (inter-molecular complex). Heteronuclear NMR measurements were performed and showed that the E6 protein was folded with similar conformations in both covalent and non-covalent complexes. These data open the way to novel structural and functional studies of the BPV1 E6 in complex with its preferential target motif.
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Dates and versions

hal-03049172 , version 1 (18-12-2020)

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Abdellahi Ould M’hamed Ould Sidi, Khaled Ould Babah, Nicole Brimer, Yves Nominé, Christophe Romier, et al.. Strategies for bacterial expression of protein–peptide complexes: Application to solubilization of papillomavirus E6. Protein Expression and Purification, 2011, 80 (1), pp.8-16. ⟨10.1016/j.pep.2011.06.013⟩. ⟨hal-03049172⟩
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