Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 2022

Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway

Résumé

Pseudomonas aeruginosa is one of the most important nosocomial bacteria emerging as a highly multidrug-resistant pathogen. P. aeruginosa produces two siderophores including pyochelin to fulfill its need for iron during infections. We know that both outer and inner membrane proteins FptA and FptX are involved in the ferri-pyochelin uptake, but this process requires increasing molecular and biochemical knowledge. Here, using bacterial two-hybrid and copurification assays we identified the fatty acid coenzyme-A ligase FadD1 as a novel interacting partner of the inner membrane transporter FptX, and found that FadD1 may play a role in pyochelin production. We managed to purify the FadD1-FptX inner membrane complex and obtained low-resolution 3D models, opening the way for future high-resolution structures.
Fichier principal
Vignette du fichier
FEBSLett 2020 Accepted.pdf (2.85 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03039826 , version 1 (04-12-2020)

Identifiants

Citer

Béatrice Roche, Gaëtan L.A. Mislin, Isabelle J Schalk. Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway. FEBS Letters, inPress, ⟨10.1002/1873-3468.14012⟩. ⟨hal-03039826⟩

Collections

CNRS SITE-ALSACE
14 Consultations
13 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More