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Article Dans Une Revue Neurogastroenterology & Motility Année : 2020

Endogenous control of inflammatory visceral pain by T cell‐derived opioids in IL‐10‐deficient mice

Résumé

Background: The opioid-mediated analgesic activity of mucosal CD4+ T lymphocytes in colitis has been reported in immunocompetent mice so far. Here, we investigated whether CD4+ T lymphocytes alleviate from inflammation-induced abdominal pain in mice with defective immune regulation. Methods: Endogenous control of visceral pain by opioids locally produced in inflamed mucosa was assessed in IL-10-deficient mice. Key results: CD4+ T lymphocytes but not F4/80+ macrophages isolated from the lamina propria of IL-10-deficient mice with colitis express enkephalin-containing opioid peptides as assessed by cytofluorometry. Colitis in IL-10-/- mice was not associated with abdominal pain. Intraperitoneal injection of naloxone-methiodide, a peripheral opioid receptor antagonist, induced abdominal hypersensitivity in IL-10-/- mice with colitis. Conclusion and inferences: Opioid-mediated analgesic activity of mucosal T lymphocytes remains operating in IL-10-/- mice with impaired immune regulation. The data suggest that endogenous T cell-derived opioids might reduce inflammation-induced abdominal pain in inflammatory bowel diseases associated with homozygous "loss of function mutations" in interleukin-10.
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hal-03026676 , version 1 (30-11-2020)

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Lilian Basso, Mehdi Benamar, Xavier Mas‐orea, Céline Deraison, Catherine Blanpied, et al.. Endogenous control of inflammatory visceral pain by T cell‐derived opioids in IL‐10‐deficient mice. Neurogastroenterology & Motility, 2020, 32 (2), 9 p. ⟨10.1111/nmo.13743⟩. ⟨hal-03026676⟩
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