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Article Dans Une Revue Development, growth & differentiation. Année : 2013

Practical tips for imaging ascidian embryos

Résumé

Decades of studies on ascidian embryogenesis have culminated in deciphering the first gene regulatory "blueprint" for the generation of all major larval tissue types in chordates. However, the current gene regulatory network (GRN) is not well integrated with the morphogenetic and cellular processes that are also taking place during embryogenesis. Describing these processes represents a major ongoing challenge, aided by recent advances in imaging and fluorescent protein (FP) technologies. In this report, we describe the application of these technologies to the developmental biology of ascidians and provide a detailed practical guide on the preparation of ascidian embryos for imaging.
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Dates et versions

hal-03026059 , version 1 (16-12-2020)

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Takefumi Negishi, Alex Mcdougall, Hitoyoshi Yasuo. Practical tips for imaging ascidian embryos. Development, growth & differentiation., 2013, 55 (4), pp.446-453. ⟨10.1111/dgd.12059⟩. ⟨hal-03026059⟩
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