Sperm-Induced Currents at Fertilization in Sea Urchin Eggs Injected with EGTA and Neomycin - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Developmental Biology Année : 1992

Sperm-Induced Currents at Fertilization in Sea Urchin Eggs Injected with EGTA and Neomycin

Karl Swann
  • Fonction : Auteur
David Mcculloh
  • Fonction : Auteur
Edward Chambers
  • Fonction : Auteur
Michael Whitaker
  • Fonction : Auteur

Résumé

Membrane currents were measured in single voltage-clamped sea urchin eggs (Lytechinus pi&us and Lytechinus variegates) that were injected with either EGTA or neomycin and inseminated. Although egg activation and the fertilization calcium wave were prevented by injection of either of these compounds, sperm attached and still elicited inward currents. Sperm-induced currents in EGTA-injected eggs had an abrupt onset, quickly reached a maximum, and then slowly declined in amplitude. Sperm incorporation occurred readily in EGTA-injected eggs. Similar results were obtained with another calcium chelator, BAPTA. In neomycin-injected eggs, sperm-induced currents generally had an abrupt onset and, in contrast to EGTA-injected eggs, the currents usually cut off rapidly. Sperm failed to enter the neomycin-injected eggs and the duration of sperm-induced currents in neomycin-injected eggs was markedly dependent upon the voltage-clamp holding potential, with shorter duration currents occurring at-70 than at-20 mV. The lability of the initial interaction between sperm and egg at negative holding potentials may explain why activation often fails when the egg membrane is voltage clamped at these potentials (
Fichier principal
Vignette du fichier
1992 SwannEGTAneomycin1992.pdf (3.76 Mo) Télécharger le fichier
Origine : Accord explicite pour ce dépôt

Dates et versions

hal-03025613 , version 1 (16-12-2020)

Identifiants

Citer

Karl Swann, David Mcculloh, Alex Mcdougall, Edward Chambers, Michael Whitaker. Sperm-Induced Currents at Fertilization in Sea Urchin Eggs Injected with EGTA and Neomycin. Developmental Biology, 1992, 151 (2), pp.552-563. ⟨10.1016/0012-1606(92)90193-K⟩. ⟨hal-03025613⟩
22 Consultations
37 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More