Article Dans Une Revue Cellular Signalling Année : 2001

Cyclic GMP-dependent protein kinase potentiates serotonin-induced Egr-1 binding activity in PC12 cells

Résumé

The NO/cyclic GMP (cGMP) signal transduction pathway, which involves the cGMP-dependent protein kinase (PKG), regulates transcription of several genes, including immediate early genes. Using transfection experiments with the PKG-Ia cDNA cloned from human aorta, we show here that addition of membrane-permeable cGMP analogues to PC12 cells slightly upregulated ERK MAP (mitogen-activated protein) kinase. Likewise, PKG-Ia was found to activate weakly DNA binding activity of the Egr-1 transcription factor. On the other hand, PKG-Ia overexpression was shown to tremendously amplify the Egr-1 binding activity induced by the neurotransmitter serotonin, which activates egr-1 gene expression also via the stimulation of the ERK MAP kinase pathway.

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hal-03021318 , version 1 (24-11-2020)

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Laurent Esteve, Pierre Lutz, Nathalie Thiriet, Marie-Odile Revel, Dominique Aunis, et al.. Cyclic GMP-dependent protein kinase potentiates serotonin-induced Egr-1 binding activity in PC12 cells. Cellular Signalling, 2001. ⟨hal-03021318⟩
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