Non-indigenous crayfish species are now more numerous than indigenous ones in Europe. Escaped from aquaria in southern Germany in the late 1990’s, Calico Crayfish (Orconectes immunis) is actually spreading in the North Eastern part of France through the Upper Rhine Valley. Once in a waterbody, it can quickly develop a massive population that turn the ecosystem into a “milky coffee” water system, leading to the vegetation disappearance and a strong reduction of amphibian and macroinvertebrate communities. Orconectes immunis also digs deep burrows, which allow it to occupy shallow and temporary water bodies and colonize a wide range of alluvial ecosystems. It has been recorded since 2013 in the Woerr “Espace Naturel Sensible”, an alluvial protected site hosting a former gravel pit, a former river channel and several restored ponds and waterbodies. We here documented some biological traits of this species in its new distribution area, especially its abilities to withstand drawdown periods through desiccation survival capacities, abilities to disperse through terrestrial habitats and dig burrows in different sediment types. Interactions between Calico crayfish and macrophytes used in restoration technics were also studied. We demonstrated that (i) some plant species grown up on floating beds can survive a Calico crayfish invasion and (ii) that the crayfish avoids coarse sediments. We can therefore suggest some preventive measures to limit this species invasion.