Article Dans Une Revue Marine drugs Année : 2017

Does Osmotic Stress Affect Natural Product Expression in Fungi?

David Overy
  • Fonction : Auteur
Hebelin Correa
  • Fonction : Auteur
Wei-Chiung Chi
  • Fonction : Auteur
Ka-Lai Pang
  • Fonction : Auteur
Mostafa Rateb
  • Fonction : Auteur
Rainer Ebel
  • Fonction : Auteur
Zhuo Shang
  • Fonction : Auteur
Rob Capon
  • Fonction : Auteur
Gerald Bills
  • Fonction : Auteur

Résumé

The discovery of new natural products from fungi isolated from the marine environment has increased dramatically over the last few decades, leading to the identification of over 1000 new metabolites. However, most of the reported marine-derived species appear to be terrestrial in origin yet at the same time, facultatively halo- or osmotolerant. An unanswered question regarding the apparent chemical productivity of marine-derived fungi is whether the common practice of fermenting strains in seawater contributes to enhanced secondary metabolism? To answer this question, a terrestrial isolate of Aspergillus aculeatus was fermented in osmotic and saline stress conditions in parallel across multiple sites. The ex-type strain of A. aculeatus was obtained from three different culture collections. Site-to-site variations in metabolite expression were observed, suggesting that subculturing of the same strain and subtle variations in experimental protocols can have pronounced effects upon metabolite expression. Replicated experiments at individual sites indicated that secondary metabolite production was divergent between osmotic and saline treatments. Titers of some metabolites increased or decreased in response to increasing osmolite (salt or glycerol) concentrations. Furthermore, in some cases, the expression of some secondary metabolites in relation to osmotic and saline stress was attributed to specific sources of the ex-type strains.

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hal-03015848 , version 1 (27-05-2024)

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David Overy, Hebelin Correa, Catherine Roullier, Wei-Chiung Chi, Ka-Lai Pang, et al.. Does Osmotic Stress Affect Natural Product Expression in Fungi?. Marine drugs, 2017, 15 (8), pp.254. ⟨10.3390/md15080254⟩. ⟨hal-03015848⟩
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