Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2020

Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process

Benjamin Petre
Mauricio Contreras
  • Fonction : Auteur
Tolga Bozkurt
  • Fonction : Auteur
Martin Schattat
  • Fonction : Auteur
Jan Sklenar
  • Fonction : Auteur
Sebastian Schornack
  • Fonction : Auteur
Ahmed Abd-El-Haliem
  • Fonction : Auteur
Roger Castells-Graells
  • Fonction : Auteur
Rosa Lozano-Duran
  • Fonction : Auteur
Yasin Dagdas
  • Fonction : Auteur
Frank Menke
  • Fonction : Auteur
Alexandra Jones
  • Fonction : Auteur
Jack Vossen
Silke Robatzek
  • Fonction : Auteur
Sophien Kamoun
Joe Win

Résumé

Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically function by associating with host molecules and modulating their activities. This study aimed to identify the host processes targeted by the RXLR class of host-translocated effectors of the potato blight pathogen Phytophthora infestans. To this end, we performed an in planta protein-protein interaction screen by transiently expressing P. infestans RXLR effectors in Nicotiana benthamiana leaves followed by co-immunoprecipitation (co-IP) and liquid chromatography tandem mass spectrometry (LC-MS/MS). This screen generated an effector-host protein interactome matrix of 59 P. infestans RXLR effectors x 586 N. benthamiana proteins. Classification of the host interactors into putative functional categories revealed over 35 biological processes possibly targeted by P. infestans. We further characterized the PexRD12/31 family of RXLR-WY effectors, which associate and co-localize with components of the vesicle trafficking machinery. One member of this family, PexRD31, increased the number of FYVE positive vesicles in N. benthamiana cells. FYVE positive vesicles also accumulated in leaf cells near P. infestans hyphae, indicating that the pathogen may enhance endosomal trafficking during infection. We anticipate that the interactome dataset we generated will serve as a useful community resource for functional studies of P. infestans effectors and of effector-targeted host processes.

Dates et versions

hal-03015488 , version 1 (19-11-2020)

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Benjamin Petre, Mauricio Contreras, Tolga Bozkurt, Martin Schattat, Jan Sklenar, et al.. Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process. 2020. ⟨hal-03015488⟩
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