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Pré-Publication, Document De Travail Année : 2020

Live imaging and biophysical modeling support a button-based mechanism of somatic homolog pairing in Drosophila

Myron Child
  • Fonction : Auteur
Jack R Bateman
  • Fonction : Auteur
Amir Jahangiri
  • Fonction : Auteur
Armando Reimer
  • Fonction : Auteur
Nicholas Lammers
  • Fonction : Auteur
Nica Sabouni
  • Fonction : Auteur
Diego Villamarin
Grace Mckenzie-Smith
  • Fonction : Auteur
Justine Johnson
  • Fonction : Auteur
Hernan Garcia
  • Fonction : Auteur

Résumé

The spatial configuration of the eukaryotic genome is organized and dynamic, providing the structural basis for regulated gene expression in living cells. In Drosophila melanogaster , 3D genome organization is characterized by somatic homolog pairing, where homologous chromosomes are intimately paired from end to end; however, the process by which homologs identify one another and pair has remained mysterious. A recent model proposed that specifically interacting “buttons” encoded along the lengths of homologous chromosomes drive somatic homolog pairing. Here, we turned this hypothesis into a precise biophysical model to demonstrate that a button-based mechanism can lead to chromosome-wide pairing. We tested our model and constrained its free parameters using live-imaging measurements of chromosomal loci tagged with the MS2 and PP7 nascent RNA labeling systems. Our analysis showed strong agreement between model predictions and experiments in the separation dynamics of tagged homologous loci as they transition from unpaired to paired states, and in the percentage of nuclei that become paired as embryonic development proceeds. In sum, as a result of this dialogue between theory and experiment, our data strongly support a button-based mechanism of somatic homolog pairing in Drosophila and provide a theoretical framework for revealing the molecular identity and regulation of buttons.
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Dates et versions

hal-03012922 , version 1 (18-11-2020)

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Myron Child, Jack R Bateman, Amir Jahangiri, Armando Reimer, Nicholas Lammers, et al.. Live imaging and biophysical modeling support a button-based mechanism of somatic homolog pairing in Drosophila. 2020. ⟨hal-03012922⟩
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