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Article Dans Une Revue Nucleic Acids Research Année : 1989

Synthesis, thermal stability and reactivity towards 9-aminoellipticine of double-stranded oligonucleotides containing a true abasic site

Jean-Jacques Vasseur
Bernard Rayner
  • Fonction : Auteur
Jean-Louis Imbach
  • Fonction : Auteur
Jean-Rémi Bertrand
  • Fonction : Auteur
Jean-Jacques Vasseurl
  • Fonction : Auteur
Bernard Rayner
  • Fonction : Auteur
Jean-Louis Imbachl
  • Fonction : Auteur
Jacques Paoletti
  • Fonction : Auteur
Claude Paoletti
  • Fonction : Auteur
Claude Malvy
  • Fonction : Auteur
Villejuif Cedex
  • Fonction : Auteur

Résumé

A 13 mers abasic oligonucleotide was synthetized. It was therefore possible to compare thermal stability and reactivity of duplex oligonucleotides either with an apurinic/apyrimidinic site or without any lesion. An important decrease in the melting temperature appeared for duplexes with an abasic site. The chemical reaction of these modified oligonucleotides with the intercalating agent 9-aminoellipticine was studied by gel electrophoresis and by fluorescence. The formation of a Schiff base between 9-aminoellipticine and abasic sites was rapid and complete with duplexes at 11 'C. Schiff base related fluorescence and 3-elimination cleavage were more important with the apyrimidinic sites than with the apurinic ones. When compared to previous results obtained with the model d(TprpT) some unexpected behaviours appeared with longer and duplex oligonucleotides. For instance only partial ,3-elimination cleavage was observed. It is likely that stacking parameters in the double helix play a great role in the studied reaction.
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Dates et versions

hal-03008450 , version 1 (16-11-2020)

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Citer

J.J. Vasseur, Jean-Jacques Vasseur, Bernard Rayner, Jean-Louis Imbach, Jean-Rémi Bertrand, et al.. Synthesis, thermal stability and reactivity towards 9-aminoellipticine of double-stranded oligonucleotides containing a true abasic site. Nucleic Acids Research, 1989, 17 (24), pp.10307-10319. ⟨10.1093/nar/17.24.10307⟩. ⟨hal-03008450⟩
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