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Article Dans Une Revue Scientific Reports Année : 2020

Yeast Ppz1 protein phosphatase toxicity involves the alteration of multiple cellular targets

Johanna Ceschin
Claire Almyre
  • Fonction : Auteur
Benoît Pinson
Bertrand Daignan-Fornier
Diego Velázquez
  • Fonction : Auteur
Marcel Albacar
  • Fonction : Auteur
Chunyi Zhang
  • Fonction : Auteur
Carlos Calafí
  • Fonction : Auteur
María López-Malo
  • Fonction : Auteur
Javier Torres-Torronteras
  • Fonction : Auteur
Ramón Martí
  • Fonction : Auteur
Sergey Kovalchuk
  • Fonction : Auteur
Ole Jensen
  • Fonction : Auteur
Antonio Casamayor
  • Fonction : Auteur
Joaquín Ariño
  • Fonction : Auteur

Résumé

Control of the protein phosphorylation status is a major mechanism for regulation of cellular processes, and its alteration often lead to functional disorders. Ppz1, a protein phosphatase only found in fungi, is the most toxic protein when overexpressed in Saccharomyces cerevisiae. To investigate the molecular basis of this phenomenon, we carried out combined genome-wide transcriptomic and phosphoproteomic analyses. We have found that Ppz1 overexpression causes major changes in gene expression, affecting ~ 20% of the genome, together with oxidative stress and increase in total adenylate pools. Concurrently, we observe changes in the phosphorylation pattern of near 400 proteins (mainly dephosphorylated), including many proteins involved in mitotic cell cycle and bud emergence, rapid dephosphorylation of Snf1 and its downstream transcription factor Mig1, and phosphorylation of Hog1 and its downstream transcription factor Sko1. Deletion of HOG1 attenuates the growth defect of Ppz1-overexpressing cells, while that of SKO1 aggravates it. Our results demonstrate that Ppz1 overexpression has a widespread impact in the yeast cells and reveals new aspects of the regulation of the cell cycle.

Dates et versions

hal-02992958 , version 1 (06-11-2020)

Identifiants

Citer

Christelle Saint-Marc, Johanna Ceschin, Claire Almyre, Benoît Pinson, Bertrand Daignan-Fornier, et al.. Yeast Ppz1 protein phosphatase toxicity involves the alteration of multiple cellular targets. Scientific Reports, 2020, 10 (1), pp.15613. ⟨10.1038/s41598-020-72391-y⟩. ⟨hal-02992958⟩

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