The aqueduct of Nicaea (modern Iznik, in northwestern Turkey) was studied for the first time using combined stratigraphical and geophysical methods. The analysis of the different materials and building techniques used allowed us to individualize more than forty stratigraphical units on the section investigated, using thirteen specific techniques. The comparison of certain masonries with analogous techniques visible in the defensive walls of the city and our stratigraphical interpretations led us to propose a chronology of the construction divided into nine phases. Some of these rebuildings seem linked to war and earthquake damage. The aqueduct was originally built in the first centuries AD using a framework of terracottas and limestone rubble. Later on, two functional terracotta structures were added and the specus was extensively rebuilt. In a second period, the early facing was replaced by well-cut travertines. Significant rebuilding occurred around the 11 th century when the city went under attacks with the Turks. The last modifications date from the Lascarid period and are probably linked to the construction of a second defensive wall in the 13 th century, which cuts the western end of the aqueduct. Geophysical acquisitions on the eastern section of the aqueduct evidenced a vertical offset of the building located on an active normal fault unsuspected before. This kind of multidisciplinary approaches are powerful tools to study active tectonics and their impact on past societies.