Genotoxic damages in zebrafish submitted to a polymetallic gradient displayed by the Lot River (France)
Résumé
Genotoxic effects of a polymetallic pollution gradient displayed by the Lot River and one of its tributary have been assessed on zebrafish Danio rerio. Three methods were compared: RAPD-PCR, the comet assay, and 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) formation. The fishes were exposed for 14 days to waters collected from three stations: Joanis, a site polluted by cadmium (Cd) and zinc (Zn) (mean concentrations: 15. γg Cd/L and 550. γg Zn/L), Bouillac (mean concentrations: 0.55. γg Cd/L and 80. γg Zn/L), and Boisse-Penchot, a reference station (mean concentrations:<0.05. γg Cd/L and 7. γg Zn/L). The quantitative RAPD-PCR methodology proved to be sensitive enough to unmask metal genotoxicity after 3 and 7 days of exposure to Joanis water and after 14 days to Bouillac water, whereas the comet assay only detected DNA damages at the most contaminated station (Joanis). The 8-oxodG quantification was not sensitive enough to be used in zebrafish under these environmental conditions. © 2011 Elsevier Inc.
Mots clés
8 hydroxydeoxyguanosine
cadmium
metal
zinc
assay
bioaccumulation
cyprinid
ecotoxicology
genotoxicity
pollutant source
pollution effect
pollution exposure
polymerase chain reaction
quantitative analysis
animal tissue
article
comet assay
concentration (parameters)
controlled study
DNA damage
environmental exposure
France
intermethod comparison
nonhuman
random amplified polymorphic DNA
river
sensitivity analysis
water pollution
zebra fish
Lot River
Danio rerio
Pisces