Using 13C to Quantify Phloem Transport on Tall Plants in the Field
Résumé
The difference in time lags between a labeling pulse of (13) CO2 of the foliage and the appearance of labeled C in the respiration at different locations along the stem of a tall plant is used to estimate at which velocities the isotope tracer, i.e., the labeled carbohydrates, are transported in the phloem sap. Here we describe a method for pulse-labeling tall plants in the field and subsequently tracing C-13 in the respiratory efflux of CO2.