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Article Dans Une Revue Bio-protocol Année : 2017

Optogenetic Stimulation and Recording of Primary Cultured Neurons with Spatiotemporal Control

Résumé

We studied a network of cortical neurons in culture and developed an innovative optical device to stimulate optogenetically a large neuronal population with both spatial and temporal precision. We first describe how to culture primary neurons expressing channelrhodopsin. We then detail the optogenetic setup based on the workings of a fast Digital Light Processing (DLP) projector. The setup is able to stimulate 10 to ~100 neurons with independent trains of light pulses that evoked action potentials with high temporal resolution. During photostimulation, network activity was monitored using patch-clamp recordings of up to 4 neurons. The experiment is ideally suited to study recurrent network dynamics or biological processes such as plasticity or homeostasis in a network of neurons when a sub-population is activated by distinct stimuli whose characteristics (correlation, rate, and, size) were finely controlled.

Domaines

Neurobiologie
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Dates et versions

hal-02942471 , version 1 (28-09-2021)

Identifiants

Citer

Jérémie Barral, Alex Reyes. Optogenetic Stimulation and Recording of Primary Cultured Neurons with Spatiotemporal Control. Bio-protocol , 2017, 7 (12), ⟨10.21769/BioProtoc.2335⟩. ⟨hal-02942471⟩
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