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Journal Articles Processes Year : 2020

Generic Model Control Applied to E. coli BL21(DE3) Fed-Batch Cultures

Laurent Dewasme
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  • PersonId : 1077342
Sihem Tebbani
Didier Dumur
Alain Vande Wouwer
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  • PersonId : 1077343

Abstract

This work proposes a Generic Model Control (GMC) strategy to regulate biomass growth in fed-batch cultures of Escherichia coli BL21(DE3). The control law is established using a previously validated mechanistic model based on the overflow metabolism paradigm. A model reduction is carried out to prevent the controller from relying on kinetics, which may be uncertain. In order to limit the controller to the use of a single measurement, i.e., biomass concentration which is readily available, a Kalman filter is designed to reconstruct the nonmeasurable information from the outlet gas and the remaining stoichiometry. Several numerical simulations are presented to assess the controller robustness with respect to model uncertainty. Experimental validation of the proposed GMC strategy is achieved with a lab-scale bioreactor.
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Dates and versions

hal-02941251 , version 1 (16-09-2020)

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Merouane Abadli, Laurent Dewasme, Sihem Tebbani, Didier Dumur, Alain Vande Wouwer. Generic Model Control Applied to E. coli BL21(DE3) Fed-Batch Cultures. Processes, 2020, 8 (7), pp.772. ⟨10.3390/pr8070772⟩. ⟨hal-02941251⟩
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