Influence of the reducing-end anomeric configuration of the Man 9 epitope on DC-SIGN recognition - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Organic & Biomolecular Chemistry Année : 2020

Influence of the reducing-end anomeric configuration of the Man 9 epitope on DC-SIGN recognition

Résumé

High-mannose (Man9GlcNAc2) is the main carbohydrate unit present in viral envelope glycoproteins such as gp120 of HIV and the GP1 of Ebola virus. This oligosaccharide comprises the Man9 epitope conjugated to two terminal N-acetylglucosamines by otherwise rarely-encountered β-mannose glycosidic bond. Formation of this challenging linkage is the bottleneck of the few synthetic approaches described to prepare high mannose. Herein, we report the synthesis of the Man9 epitope with both alpha and beta configurations at the reducing end, and subsequent evaluation of the impact of this configuration on binding to natural receptor of high-mannose, DC-SIGN. Using fluorescence polarization assays, we demonstrate that both anomers bind to DC-SIGN with comparable affinity. These relevant results therefore indicate that the more synthetically-accesible Man9 alpha epitope may be deployed as ligand for DC-SIGN in both in vitro and in vivo biological assays.

Dates et versions

hal-02934470 , version 1 (09-09-2020)

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Citer

Noelia de La Cruz, Javier Ramos-Soriano, José Reina, José de Paz, Michel Thépaut, et al.. Influence of the reducing-end anomeric configuration of the Man 9 epitope on DC-SIGN recognition. Organic & Biomolecular Chemistry, 2020, 18 (31), pp.6086-6094. ⟨10.1039/d0ob01380c⟩. ⟨hal-02934470⟩
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