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Article Dans Une Revue Genes and Development Année : 2012

A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole

Résumé

The cell poles constitute key subcellular domains that are often critical for motility, chemotaxis, and chromosome segregation in rod-shaped bacteria. However, in nearly all rods, the processes that underlie the formation, recognition, and perpetuation of the polar domains are largely unknown. Here, in Vibrio cholerae, we identified HubP (hub of the pole), a polar transmembrane protein conserved in all vibrios, that anchors three ParA-like ATPases to the cell poles and, through them, controls polar localization of the chromosome origin, the chemotactic machinery, and the flagellum. In the absence of HubP, oriCI is not targeted to the cell poles, chemotaxis is impaired, and a small but increased fraction of cells produces multiple, rather than single, flagella. Distinct cytoplasmic domains within HubP are required for polar targeting of the three ATPases, while a periplasmic portion of HubP is required for its localization. HubP partially relocalizes from the poles to the mid-cell prior to cell division, thereby enabling perpetuation of the polar domain in future daughter cells. Thus, a single polar hub is instrumental for establishing polar identity and organization.

Domaines

Bactériologie
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Dates et versions

hal-02929596 , version 1 (07-12-2022)

Identifiants

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Yoshiharu Yamaichi, Raphael Bruckner, Simon Ringgaard, Andrea Möll, D Ewen Cameron, et al.. A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole. Genes and Development, 2012, 26 (20), pp.2348-2360. ⟨10.1101/gad.199869.112⟩. ⟨hal-02929596⟩
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